In this study, we have compared the effects of sub-lethal concentrations of clothianidin (Supertox 48% SC) and Spinoza (Tracer 24% SC) on mortality percent, brood lock, C-shape and foraging behavior of first Carniola and Italian hybrids honeybee larvae in hive. The ingestion food was contaminated with the tested insecticides at concentrations of 0.00, 0.001, 0.005, 0.01, 0.05 and 0.1 ppm. Results revealed that the two tested pesticides at hive-residue levels triggered increase in larval mortality according to untreated larvae after 7 days of exposure. Moreover, hybrids Carniola and Italian honeybees' larvae were more sensitive to Spinosad compared with 88.89% and 75.00% mortality after 7 days, respectively, while the corresponding values with clothianidin were 72.22% and 50.00% for both hybrids honeybee' larvae, respectively.