The goal of the present work was to investigate the influence of laser irradiation on in vitro and in vivo growth agro-morphological criteria, mitotic activities and the chromosomes on Atropa belladonna L. The influence of laser radiation on Atropa seeds was investigated using helium–neon (He–Ne) were exposed to levels of laser doses (0.0,10,15, 20, 25 and 30 J cm)^-2 using 7 m W He–Ne laser (632.8 nm),with a power density of 4.02 m W cm)².The most of morphological, floral parameters germination   percentage, plant height (cm), no. of leaves per plant, no. of branches per plant, leaf Length, width of leaf, leaf area and root length were studied in, First, Second and third months. The results showed significant differences among the five doses of laser radiation on seed germination, seedling height, plant height, no. of Leaves per plant at 10,20 and 30 days. The best doses 25 Jcm^-2 to have a stimulating effect on these traits. The cytogenetic effects of physical mutagens Laser beam (0.0,10,15, 20, 25 and 30 J cm)^-2were investigated on the root tip cells of Atropa belladonna L., Various types of chromosomal aberrations (multinucleate cells) were observed during the cytological analysis of the root tip cells of both treatments. On the other hand, the high doses caused significant reduction as comparing with the control for all studied traits.