Microbial degradation is the main process controlling the environmental dissipation of the nematicides. Oxamyl is an important Carbamate nematicide that is used for the control of nematodes in many economic crops in Egypt. Out of   twelve bacterial isolates which were isolated from different Egyptian soils and showed high ability to grow and tolerate 40 ppm oxamyl as compared with other isolates, two bacterial isolates were found to be the most active decomposers of oxamyl in MSL medium, They were identified as Brevibacillus gelatini OX₁MZ265175.1and Brevundimonas diminuta OX₂ MZ265176.1 based on morphological, biochemical characterization and 16S r DNA gene sequencing,. The environmental factors Affecting the bacterial growth and biodegradation of oxamyl at 40ppm, i.e.; temperature degrees, pH values and carbon and nitrogen sources were studied. The two identified strains were varied in their response to the different environmental factors. The determination of oxamyl degradation was performed using HPLC technique.