Biotechnology has contributed to the economic prosperity of the outside world. One of the major areas of biotechnology is tissue and plant cell culture. And useful in the rapid propagation of plants. The banana crop is one of the most important horticultural corps in which tissue culture technology is used. The area cultivated with tissue culture technology reached about 21.4 thousand acre. As an average for the period (2016-2018). The research aimed to highlight the role of technological development in the biological field and its importance in raising the production efficiency of the banana crop using tissue culture technology. In achieving its objectives, the research relied on using the method of quantitative and descriptive analysis, and measuring some indicators of productive and economic efficiency. The research also relied on field data for a random sample of 200 individuals from the two study areas (Qalyubia Governorate and Nub aria region) during the 2019/2018 agricultural season. It was evident from the results obtained that the banana farmers produced with tissue culture technology achieved a net profit of about 112 thousand pounds / acre. An increase of about 60 thousand pounds / acre. On the net profit of banana farmers produced in the traditional way, which amounted to about 52 thousand pounds / acre. It was also revealed through the use of the analysis of variance that there is a significant difference between the method of tissue culture technique and the traditional method of growing banana crop. Recommendations: The necessity of introducing modern biological technologies in the agricultural sector to raise productivity, and improving the quality of agricultural products and maximize the economic return.