Beta
218197

Effect of chitosan and light conditions on the production of callus biomass, total flavonoids and total phenolics in Ginkgo biloba L.

Article

Last updated: 04 Jan 2025

Subjects

-

Tags

-

Abstract

Ginkgo biloba L. is a well-known tree for its high medicinal value owing to the presence of terpene trilactones, unique active metabolites, and other phytochemicals, including flavonoids and phenolics in its leaves. In Egypt, G. biloba has been reported as one of the endangered trees. Hence, plant tissue technique could be a potential alternative system to produce ginkgo's pharmaceutical compounds. In this study, an elicitation strategy using the biotic elicitor chitosan was employed to enhance the productivity of certain metabolites in G. biloba leaf-derived callus incubated in light (16 h photoperiod) or darkness. Combination of 1.0 mg/L α-naphthalene acetic acid (NAA) and 2.0 mg/L 6-benzyl adenine (BA) added to Murashige and Skoog (MS) medium resulted in the highest callogenesis percentage in both light and dark (95.00 and 94.43%, respectively) with a compacted callus texture but a different color. Significant differences were recorded between chitosan levels concerning callus biomass production and total flavonoids and phenolics accumulation. The highest yield of callus fresh and dry weights, growth index, and relative growth rate were recorded for light-grown cultures elicited with 100 mg/L chitosan followed by 50 mg/L. Same concentrations were also superior in enhancing the content and productivity of total flavonoids and total phenolics. Light incubation was more favorable than darkness for callus growth, flavonoid, and phenolic biosynthesis as well as antioxidant activity. The current study revealed the possibility of using calli culture as a biotechnological approach for the industrial production of G. biloba phytoconstituents.

DOI

10.21608/ajar.2021.218197

Keywords

Ginkgo biloba, Chitosan, biotic elicitation, Flavonoids, Phenolics, Antioxidant activity, Callus culture, light photoperiod

Authors

First Name

Ahmed

Last Name

Elateeq

MiddleName

Abdelfattah

Affiliation

Horticulture Department, Faculty of Agriculture, Al-Azhar University, Nasr City, Cairo, Egypt

Email

ahmedelateeq@azhar.edu.eg

City

Cairo

Orcid

0000-0002-9417-2684

First Name

Zakaryia

Last Name

Saad

MiddleName

-

Affiliation

Department of Biochemistry, Faculty of Agriculture, Al-Azhar University, Nasr City, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

M.

Last Name

Eissa

MiddleName

-

Affiliation

Biotechnology Department, Faculty of Agriculture, Al-Azhar University, Nasr City, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

S.

Last Name

Ullah

MiddleName

-

Affiliation

Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin, China

Email

-

City

-

Orcid

-

Volume

46

Article Issue

1

Related Issue

31069

Issue Date

2021-06-01

Receive Date

2021-04-07

Publish Date

2021-06-01

Page Start

28

Page End

42

Print ISSN

1110-1563

Online ISSN

2786-0051

Link

https://ajar.journals.ekb.eg/article_218197.html

Detail API

https://ajar.journals.ekb.eg/service?article_code=218197

Order

3

Type

Original Article

Type Code

929

Publication Type

Journal

Publication Title

Al-Azhar Journal of Agricultural Research

Publication Link

https://ajar.journals.ekb.eg/

MainTitle

Effect of chitosan and light conditions on the production of callus biomass, total flavonoids and total phenolics in Ginkgo biloba L.

Details

Type

Article

Created At

22 Jan 2023