This study was conducted to investigate the effect of surgical excision of the uropygial gland at 14 and 28 d of age on some physiological characteristics of Ross broiler chicks. a total number of 270 day-old birds were randomly distributed into 3 treatments, 90 birds/treatment, T₁: control without surgical excision of the uropygial gland. Treatment T₂: surgical excision of the uropygial gland at the age of 14 days. Treatment T₃: surgical excision of the uropygial gland was done at the age of 28 d. At the age of 6 weeks (42 d) samples of blood were withdrawn from 12 birds/treatment to measure blood traits and some serum biochemical tests.The results of T₃ showed significant increase (P£0.05) in RBCs count, Hb, PCV%, H/L ratio. Also, T₃ showed a significant increase (P£0.05) in serum corticosterone, blood glucose, uric acid, total cholesterol, AST and ALT. Furthermore, results of T₂ displayed that no significant differences (P£0.05) in corticosterone and blood glucose levels, but showed a significant increase (P£0.05) in total protein level and increased efficacy of ALP enzyme. Likewise, obtained results from T₂ offered that a significant decrease (P£0.05) in the level of uric acid and total cholesterol, in the same direction, the liver enzyme activities (AST, ALT) were significantly (P£0.05) decreased. In conclusion, the removal of uropygial gland at the age of 14 d can improve the qualities of blood and help to increase the rate of metabolism and protein synthesis, which may help to increase the rate of growth contrary to the results of removal at the age of 28 d.