A field experiment was carried out on tomato plants (Solanum lycopersicum L.)  during summer seasons of 2020 and 2021 at the experimental farm of the Faculty of Agriculture, Benha University, Moshtohor, Touch, Kalubia Governorate, Egypt, in order to investigate the response of genotypes, three tomato genotype (Alia 123 F1, Arwa F1 and Super strain B) to deficit irrigation and foliar application with stimulate and their interaction on yield, fruit qualityand water use efficiency of tomato plants grown under drip irrigation system in clay soil conditions. Obtained results showed that treatments that received 80% WR + amino acids as foliar spraying of Alia 123 recorded superior effects on early yield, marketable and total yield. The highest water use efficiency was recorded when using 80% WR and the foliar application of amino for Alia 123 cultivar with significant deferent as compared with all other treatments. Irrigation with 60% of the WR with the foliar application of any of the used foliar sprays i.e. amino, humic and calcium+boron on any of the used genotypes resulted the highest increases in TSS of tomato fruits. The same trend was found with the 100% of WR on vitamin C. highest acidity was found with 60% of WR with calcium boron or deionized water as a spray on super strain B with significant variations in the second growing season.