A pot experiment was carried out at Agricultural Research Station, Agricultural Research Center, Giza Governorate, Egypt, (latitude 26^o 31′N and longitude 31^o 11′E) during 2019 and 2020 seasons to compare two sugarcane promising clones, with the commercial variety GT.54-9 (Saccharum spp. L.), under three irrigation water levels (60, 80 and 100% of IWL). A randomized completely design with five replications was used. Some growth traits such as shoot and root FW, root/shoot ratio, leaf area index (LAI), leaf area ratio (LAR), as well as the biochemical constituents like chlorophylls, carotenoids, proline, total soluble sugars, total amino acids, total soluble proteins and macronutrients such as.. N, P, K, Mg and Ca were assessed.               Results showed that, clone 2 had the desirable values for the studied traits more than commercial variety GT.54-9, while the clone1 recorded undesirable values for the studied traits compared to GT.54-9 under water deficit stress that be contributed. So could be select the clone 2 as anew promising variety in sugar cane cultivar with drought tolerance. In addition to, sugarcane studied clones not affected by increase the degree of IWL from 80 to 100%.