In vitro osmotic stress screening of plant genotypes is a useful tool that can be used instead of field trials and is based on osmotic stress tolerance. The major purpose of this work was to employ in vitro screening to reveal the variation in potato cultivars for salt stress tolerance (10, 30, 50, 70, 100 and 120 mM NaCl). Murashige and Skoog (MS) media supplemented with these concentrations of NaCl was used to culture single node stem cuttings of eight potato cultivars (Gizela, Sponta, Cara, Kaspar, Burren, Lady Balfour, Diamant, and Maritienia). Significant differences among treatments were noticed the highest concentration of NaCl gave the lowest values for most the study characters. Moreover, there were significant differences response among cultivars for NaCl concentrations. Low concentrations of NaCl gave values close to the control treatment. The highest values of the most study characters found with Diamant cultivar treated with 120 mM NaCl compared to another cultivars.