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152729

Partial Characterisation of Two Isolates of Cucumber mosaic virus (CMV)

Article

Last updated: 22 Jan 2023

Subjects

-

Tags

Plant pathology

Abstract

Field observation revealed that the most common symptoms on naturally infected plants in Alexandria, Dakahliya, Kafr El-sheikh and El- Beheira governorates in Egypt were severe mosaic, mild mosaic, chlorosis, mottling, vein banding, blisters, malformation, fern leaf, shoe-string and/or stunting. These symptoms were suspected of being caused by Cucumber mosaic virus (CMV) and confirmed by indirect ELISA. Two samples obtained from wild tobacco and cucumber plants reacted positively with the CMV specific antiserum. Based on symptomology and disease severity on Nicotiana glutinosa and Chenopodium amaranticolor, two isolates of CMV were named CMV-wild tobacco (from Alexandria) and CMV-cucumber (from Kafr El-sheikh) and subjected to this study. Reaction of some diagnostic hosts of 11 plant species belonging to five families upon inoculation with the two isolates included different symptoms characteristic to CMV-infection, albeit those induced by CMV-wild tobacco being invariably more severe than those elicited by CMV-cucumber. Identification of virus isolates was confirmed using real time reverse transcription PCR (qRT-PCR). The test isolates demonstrated a single qRT-PCR amplification product of 500bp. The two isolates could be transmitted mechanically and easily transmitted by four aphid species in non-persistent manner. The most efficient vector was Myzus persicae followed by Aphis gossypii, Rhopalosiphum maidis and A. nerii with transmission rates of 90%, 70%, 60% and 50%, respectively, for CMV-wild tobacco and being 80%, 80%, 40% and 30%, respectively for CMV-cucumber. The two isolates could not be transmitted via Cucurbita pepo seeds derived from infected plants. However, virus infection had a great effect on seed germination.

DOI

10.21608/sjas.2021.52505.1064

Keywords

CMV, qRT-PCR, ELISA, aphids, mode of transmission

Authors

First Name

Elsayed

Last Name

Wagih

MiddleName

Elsayed

Affiliation

Plant Pathology Dept., Faculty of Agriculture, Alexandria Univ., Alexandria, Egypt.

Email

eewagih@gmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Zalat

MiddleName

Mohsen

Affiliation

Plant Pathology Dept., Faculty of Agriculture, Alexandria Univ., Alexandria, Egypt.

Email

vmmzalat@yahoo.com

City

-

Orcid

-

First Name

Maha

Last Name

Kawanna

MiddleName

Adel

Affiliation

Plant Pathology department, Faculty of Agriculture, Alexandria University

Email

mahakawanna@yahoo.com

City

-

Orcid

-

Volume

3

Article Issue

1

Related Issue

25269

Issue Date

2021-06-01

Receive Date

2020-12-06

Publish Date

2021-05-31

Page Start

144

Page End

157

Print ISSN

2535-1796

Online ISSN

2535-180X

Link

https://sjas.journals.ekb.eg/article_152729.html

Detail API

https://sjas.journals.ekb.eg/service?article_code=152729

Order

15

Type

Original Article

Type Code

916

Publication Type

Journal

Publication Title

Scientific Journal of Agricultural Sciences

Publication Link

https://sjas.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023