In an attempt to develop for high yield genotypes of bread wheat, two procedures, i.e. mutation breeding and hybridization were used to induce new genetic variations. Three field experiments were conducted during the seasons 2017/2018, 2018/2019 and 2019/2020, at the Experimental Farm, Faculty of Agriculture, Al-Azhar University Assiut Branch. The results showed that, hybridization and treated plants by 2000 ppm of hydrazine hydrate were the more effective than other treatment of hydrazine hydrate, as well as the hybrids were more responsible than the other genotypes for induction of stable promising mutants according to final results at F2 and F2M2 generation especially grain yield /plant. The F2 and M2 populations of these genotypes exhibited differences in the magnitude of phenotypic (PCV) and genotypic (GCV) coefficient of variation and heritability for studied traits under this study. The highest expected gain from selection (GA%) for grain yield/plant was obtained from Sids-14, Gemmiza 11 and Sids 14 x Giza 171 when treated by 2000 ppm from Hydrazine hydrate reached to 77.80, 57.70 and 55.71 %, respectively., more than those of the untreated F2 populations.