This study was carried out in the Animal Production Experimental farm Faculty of Agriculture, South Valley University, during the period from January to April 2018. The aim of the present study was to evaluate the effect of different dietary level of a-linolenic on the semen characteristics of the male rabbits of New Zealand White. The aim of the present study was to evaluate the effect of different dietary level of a-linolenic on the semen characteristics of the male rabbits of New Zealand White. Used Thirty New Zealand White rabbit males were divided into three groups. First group; males were fed control ration ,second group males were fed tested one ration ; (supplemented with 4% extruded flaxseed) and third group males were fed tested ration two; (supplemented with 6% extruded flaxseed). The reproductive performances and semen characteristics of the male rabbits of New Zealand White were evaluated . The diet affected many seminal traits and the males of control group showed the lowest values of live cells and spermatozoa concentration. The linoleic (18:3, n-3) fatty acids in the diet (supplemented with 4 % or 6 % extruded flaxseed) was to increased significantly their levels within the spermatozoa and to have extensive beneficial effects on parameters of spermatozoa function and therefore male fertility in the New Zealand White rabbit males