Massive outbreaks of suspected LSDV in cattle population were observed during 2017 and 2018 at Beni-Suef governorate. Samples from diseased cattle, previously SPPV vaccinated with SPPV, were inoculated in embryonated chicken eggs (ECE) using chorio-allantoic membrane (CAM) route. Histopathological examination of the inoculated CAMs showed large eosinophilic intracytoplasmic inclusion bodies characteristic for LSDV. The inoculated CAMs were hemorrhagic with congestion blood vessels appeared by the 1st passage then become more pronounced after the second - fourth passages. Characteristic pock lesions were observed after the 1st passage and become clear after the third passage and become clearly observed 4 days post inoculation. For molecular identification, DNA was extracted from a pool of the infected CAMs. Two pairs of primers specific for LSDV including one flanking a 554 bp product of the G gene and second flanking 172 of the RP030 gene were used. In conclusion, LSDV infections have been detected and the virus has been isolated and identified by PCR from cattle previously vaccinated with SPPV vaccine during the summer of 2018.