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151255

Isolation and Molecular Characterization of Marek’s Disease Virus from Layer Chickens in Egypt

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Veterinary microbiology and pathobiology (Veterinary Bacteriology & my…irology, immunology, parasitology, pathology, and molecular biology).

Abstract

Marek's disease virus is a lymphomatous neuropathic disease of domestic fowl. In Egypt, poultry flocks are suffering from recurrent uprising of MD outbreaks due to increased virulence of the virus. In this study, a total of 10 clinical samples were collected from 6-8 months old vaccinated layer chickens suffering from depression, ataxia and paralysis. These samples were examined for the presence of MDV by PCR using specific primers for ICP4 gene. MDV was detected in 5 out of 10 samples. One out of the 5 positive samples was isolated and partially sequenced for ICP4 gene (F1352-S4).  The results proved that it was genetically characterized as very virulent strain of Marek's disease virus type 1 resembling Gallid herpesvirus 2 isolates from Europe, China, Africa and India. There was a characteristic serine residue at the coding region of ICP4 gene encoding the reverse complement protein found only in the Egyptian virus (F1352-S4) and EU-1 virus, IND-KA-02-06, JL/1404 and in Debre-zeit/01/2013 virus that is tracing for the original virus CV1988. The obtained results indicated the importance of continuous monitoring of MDV infection in chicken layer flocks.

DOI

10.21608/jvmr.2021.59613.1032

Keywords

Marek’s disease virus, PCR, ICP4 gene, Egypt

Authors

First Name

Samar

Last Name

Ewies

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt

Email

samarewies@yahoo.co.uk

City

-

Orcid

0000-0001-8790-0156

First Name

Wesam

Last Name

Mady

MiddleName

-

Affiliation

National Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, P.O. Box 264-Dokki, Giza-12618, Egypt

Email

wesammady83@hotmail.com

City

cairo

Orcid

-

First Name

Enas

Last Name

Hamad

MiddleName

-

Affiliation

Animal Health Research Institute, Mansoura branch, Egypt

Email

enashamah@yahoo.com

City

-

Orcid

-

First Name

Abdelsatar

Last Name

Arafa

MiddleName

-

Affiliation

National Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, P.O. Box 264-Dokki, Giza-12618, Egypt

Email

araby85@hotmail.com

City

-

Orcid

-

First Name

Sabry

Last Name

Tamam

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt

Email

s.mtamam@yahoo.com

City

-

Orcid

0000-0002-5583-4737

First Name

Hanafy

Last Name

Madbouly

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt

Email

hmmadbouly@gmail.com

City

-

Orcid

0000-0001-9896-5182

Volume

27

Article Issue

2

Related Issue

22733

Issue Date

2020-12-01

Receive Date

2021-01-24

Publish Date

2020-12-01

Page Start

168

Page End

176

Print ISSN

2357-0512

Online ISSN

2357-0520

Article File

JVMR_Volume 27_Issue 2_Pages 168-176.pdf

PDF . 674.8kB

Link

https://jvmr.journals.ekb.eg/article_151255.html

Detail API

https://jvmr.journals.ekb.eg/service?article_code=151255

Order

7

Type

Full-length article

Type Code

893

Publication Type

Journal

Publication Title

Journal of Veterinary Medical Research

Publication Link

https://jvmr.journals.ekb.eg/

MainTitle

Isolation and Molecular Characterization of Marek’s Disease Virus from Layer Chickens in Egypt

Details

Type

Article

Created At

22 Jan 2023