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148829

Prevalence of Escherichia coli in Milk and Some Dairy Products in Beni-Suef Governorate, Egypt

Article

Last updated: 22 Jan 2023

OverviewSimilar Items

Subjects

-

Tags

Animal-derived food hygiene, safety, and technology (milk, dairy products, meat, meat products, seafood, and poultry meat).

Abstract

The aim of the study was directed to investigate the prevalence of Escherichia coli in raw milk, cream, large and small scale yoghurt, Kareish cheese, and large and small scale ice cream by conventional bacteriological methods as well as detection of the wzy gene (O-antigen polymerase gene) using polymerase chain reaction for further identification of recovered isolates. A total of 200 random samples including 40 raw milk, 40 fresh cream, 20 of each large and small scale yoghurt, 40 Kareish cheese and 20 of each large and small scale ice cream were obtained from different localities in Beni-Suef governorate, Egypt. The present study could provide useful information for the prevalence of E. coli in the examined samples: 75%, 62.5%, 0%, 25%, 80%, 10% and 25% respectively. E. coli isolate were serologically identified as: O26, O111:H4, O121, O125:H21, O169, O126:H7 and O158 in raw milk, O6:H16, O55:H7, O119:H6, O125:H6 and O146:H21 in fresh cream, O55:H7, O125:H21 in small scale yoghurt and O18, O55:H7, O114:H21, O158, O125:H21and O153:H45 in Kareish cheese. Interestingly PCR successfully amplified the wzy gene (O-antigen polymerase gene) in all E. coli isolates which is associated with LPS biosynthesis and bacterial pathogenicity and increases the ability of E. coli to withstand the anti-bacterial defense mechanism. 

DOI

10.21608/jvmr.2021.148829

Keywords

Dairy products, E. coli, Milk, PCR, serology, wzy gene

Authors

First Name

Ahmed

Last Name

Megawer

MiddleName

-

Affiliation

Milk Hygiene Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt

Email

salehmazen2016@gmail.com

City

beni-suef

Orcid

-

First Name

Gamal

Last Name

Hassan

MiddleName

-

Affiliation

Food Safety and Technology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt

Email

gamalhassen2000@yahoo.com

City

Beni suef

Orcid

-

First Name

Arafa

Last Name

Meshref

MiddleName

-

Affiliation

Food Safety and Technology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt

Email

foodhyg@yahoo.com

City

beni-suef

Orcid

-

First Name

Hala

Last Name

Elnewery

MiddleName

-

Affiliation

Bacteriology Department, Animal Health Research Institute, Beni-Suef branch, Beni-Suef, Egypt

Email

dr.hala.elnwary@gmail.com

City

beni-suef

Orcid

-

Volume

27

Article Issue

2

Related Issue

22733

Issue Date

2020-12-01

Receive Date

2020-11-26

Publish Date

2020-12-01

Page Start

161

Page End

167

Print ISSN

2357-0512

Online ISSN

2357-0520

Article File

JVMR_Volume 27_Issue 2_Pages 161-167.pdf

PDF . 876.1kB

Link

https://jvmr.journals.ekb.eg/article_148829.html

Detail API

https://jvmr.journals.ekb.eg/service?article_code=148829

Order

6

Type

Original Article

Type Code

891

Publication Type

Journal

Publication Title

Journal of Veterinary Medical Research

Publication Link

https://jvmr.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023