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146885

Prevalence, Characterization, and Control of Staphylococcus aureus Isolated from Raw Milk and Egyptian Soft Cheese

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Animal-derived food hygiene, safety, and technology (milk, dairy products, meat, meat products, seafood, and poultry meat).

Abstract

The present study aimed to detect the prevalence of Staphylococcus aureus )S. aureus( in raw milk, and soft cheese samples in Beni-Suef Governorate, Egypt, and to characterize some resistance and virulence related genes in the recovered S. aureus isolates. An additional objective was to evaluate the effectiveness of thyme oil for controlling S. aureus in cheese. A total of 200 samples of raw milk and cheese, including 100 samples of raw milk and 100 samples of two different types of cheese were used. S. aureus was isolated from 12.5% (25/200) of the raw milk and cheese samples. The highest prevalence was detected in Kareish cheese (18%), followed by raw milk samples (13%) and Talaga cheese (6%).  S. aureus isolates showed high resistance to ampicillin (72%), and tetracycline (60%). PCR was applied on 6 multidrug resistant (MDR) S. aureus for detection of mecA and icaA genes which were detected in 66.7% and 33.3% of S. aureus isolates, respectively. As milk and dairy products are good substrates for S. aureus that causes staphylococcal food poisoning, reducing the level of   such pathogen in milk and dairy products will lower the risk to consumers. Thyme oil 1% showed moderate inhibition against S. aureus, while using thyme oil 2% showed significant inhibition of S. aureus in Kareish cheese. Therefore, the use of thyme oil in preserving white soft Kareish cheese might be a promising approach to increase the shelf life and safety of the cheese.

DOI

10.21608/jvmr.2021.146885

Keywords

Antimicrobial resistance, cheese, control, Raw milk, S. aureus, Thyme oil

Authors

First Name

Mohamed

Last Name

Zeinhom

MiddleName

-

Affiliation

Food Hygiene and Control Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt

Email

m.zeinhom@vet.bsu.edu.eg

City

-

Orcid

0000-0002-0811-9941

First Name

Ahmed

Last Name

Abed

MiddleName

-

Affiliation

Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt

Email

aboabedelmasry@yahoo.com

City

Beni-Suef

Orcid

0000-0002-7221-6340

Volume

27

Article Issue

2

Related Issue

22733

Issue Date

2020-12-01

Receive Date

2021-01-06

Publish Date

2020-12-01

Page Start

152

Page End

160

Print ISSN

2357-0512

Online ISSN

2357-0520

Article File

JVMR_Volume 27_Issue 2_Pages 152-160.pdf

PDF . 974.7kB

Link

https://jvmr.journals.ekb.eg/article_146885.html

Detail API

https://jvmr.journals.ekb.eg/service?article_code=146885

Order

5

Type

Original Article

Type Code

891

Publication Type

Journal

Publication Title

Journal of Veterinary Medical Research

Publication Link

https://jvmr.journals.ekb.eg/

MainTitle

Prevalence, Characterization, and Control of Staphylococcus aureus Isolated from Raw Milk and Egyptian Soft Cheese

Details

Type

Article

Created At

22 Jan 2023