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111375

Molecular Characterization of Antimicrobial-resistant Escherichia coli Isolated from Broiler Chickens

Article

Last updated: 22 Jan 2023

OverviewSimilar Items

Subjects

-

Tags

Veterinary microbiology and pathobiology (Veterinary Bacteriology & my…irology, immunology, parasitology, pathology, and molecular biology).

Abstract

Avian colibacillosis is one of the most important diseases of chickens resulting in significant economic losses as well as high morbidity and mortality. In the current study, the prevalence of avian colibacillosis was studied in different farms of broiler chickens in Beni-Suef, EL-Minia, ElFayoum, Assiut and Sohag Governorates. A total of 300 pooling samples were collected aseptically from heart blood as well as the affected organs including air sacs, pericardial sac, and liver of slaughtered diseased and freshly dead broiler chickens. Bacteriological examination of the collected samples showed that a total number of 80 E. coli isolates were recovered with an overall prevalence of 26.7. The highest prevalence was recorded in El-Fayoum (33.3%) followed by El-Minia (25%), Beni-Suef and Assiut (22.5% for each) while the lowest prevalence was in Sohag (17.5%). The invitro antimicrobial susceptibility testing revealed that E. coli isolates showed high sensitivity to colistin sulphate only (70%). Meanwhile, high resistances were recorded against other antimicrobials including amoxicillin (97.5%), cefotaxime sodium and florfenicol (95% for each), apramycin, ciprofloxacin and gentamicin (92.5% for each), streptomycin (90%), enrofloxacin (87.5%), trimethoprim-sulphamethoxazol and doxycycline HCl (77.5% for each). All E. coli isolates were MDR (100%). PCR was applied on 10 MDR E. coli isolates to detect the 3 resistance-associated genes (qnrA, tetA and aac(6′)- Ib-cr and 5 virulence-associated genes (iutA, hly, sta, lt and astA). The results showed that all the tested isolates (100%) harbored qnrA, tetA, iutA and astA genes meanwhile aac(6′)-Ib-cr, hly, sta and lt genes were not detected in any isolate.

DOI

10.21608/jvmr.2020.31870.1009

Keywords

Broiler chickens, E. coli, Multidrug resistance (MDR), resistance genes

Authors

First Name

Ismail

Last Name

Radwan

MiddleName

-

Affiliation

Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt

Email

microbiologist111@yahoo.com

City

El Fayoum

Orcid

-

First Name

Mohammed

Last Name

Abd El-Halim

MiddleName

-

Affiliation

El-Minia Directorate of Veterinary Medicine, Egypt

Email

seifmwa@gmail.com

City

El Minia

Orcid

-

First Name

Ahmed

Last Name

Abed

MiddleName

Hussein

Affiliation

Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt

Email

aboabedelmasry@yahoo.com

City

Beni-Suef

Orcid

0000-0002-7221-6340

Volume

27

Article Issue

2

Related Issue

22733

Issue Date

2020-12-01

Receive Date

2020-06-05

Publish Date

2020-12-01

Page Start

128

Page End

142

Print ISSN

2357-0512

Online ISSN

2357-0520

Article File

JVMR_Volume 27_Issue 2_Pages 128-142.pdf

PDF . 1.3MB

Link

https://jvmr.journals.ekb.eg/article_111375.html

Detail API

https://jvmr.journals.ekb.eg/service?article_code=111375

Order

3

Type

Original Article

Type Code

891

Publication Type

Journal

Publication Title

Journal of Veterinary Medical Research

Publication Link

https://jvmr.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023