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69696

Sensitivity and specificity of Indirect Enzyme Linked Immuno sorbent Assay (ELISA) for diagnosis of hydatidosis in dromedary camels using hydatid cyst fluid antigens.

Article

Last updated: 04 Jan 2025

Subjects

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Tags

Veterinary microbiology and pathobiology (Veterinary Bacteriology & my…irology, immunology, parasitology, pathology, and molecular biology).

Abstract

Examination of 528 (450 males and 78 females) dromedary camels slaughtered at Cairo abattoir revealed that a total of (93) 17.6 % were infected with hydatidosis. Post mortem examination revealed that infection was restricted only in the lungs and the liver of infected camels. Among the 93 hydatidosis infected camels, lungs were the most frequently infected 88 (94.623%) compared with liver 5 (5.376%). ELISA test using partially crude antigen and purified antigen is important for the early diagnosis of cystic echinococcosis as most cases in the early stages of infection are asymptomatic. Sensitivity of ELISA using the crude antigen was 82.758% while sensitivity of the partially purified antigen was 79.310 %. On the other hand specificity of the crude antigen was estimated as 62.5 % and specificity of partially purified antigen as 75.0 %.

DOI

10.21608/jvmr.2020.69696

Keywords

Dromedary camels, Echinococcus granulosus, ELISA, Hydatidosis

Authors

First Name

A. M.

Last Name

El-Kattan

MiddleName

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Affiliation

Department of Animal Health Desert Research center.

Email

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City

-

Orcid

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First Name

Aml

Last Name

M. Abdel-Ra'ouf

MiddleName

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Affiliation

Department of Animal Medicine, Faculty of Veterinary Medicine, Aswan University.

Email

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City

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Orcid

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First Name

R. R.

Last Name

Yousef

MiddleName

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Affiliation

Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo. University.

Email

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City

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Orcid

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First Name

T. R.

Last Name

AbouElnga

MiddleName

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Affiliation

Department of Animal Health Desert Research center.

Email

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City

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Orcid

-

First Name

W.M.A.

Last Name

Mousa

MiddleName

-

Affiliation

Department of Parasitology, Faculty of Veterinary Medicine, Cairo University.

Email

-

City

-

Orcid

-

Volume

27

Article Issue

1

Related Issue

13956

Issue Date

2020-06-01

Receive Date

2020-02-02

Publish Date

2020-06-01

Page Start

66

Page End

75

Print ISSN

2357-0512

Online ISSN

2357-0520

Link

https://jvmr.journals.ekb.eg/article_69696.html

Detail API

https://jvmr.journals.ekb.eg/service?article_code=69696

Order

7

Publication Type

Journal

Publication Title

Journal of Veterinary Medical Research

Publication Link

https://jvmr.journals.ekb.eg/

MainTitle

Sensitivity and specificity of Indirect Enzyme Linked Immuno sorbent Assay (ELISA) for diagnosis of hydatidosis in dromedary camels using hydatid cyst fluid antigens.

Details

Type

Article

Created At

22 Jan 2023