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77992

Preparation of diagnostic ELISA kits for detection of camelpox virus

Article

Last updated: 04 Jan 2025

Subjects

-

Tags

Veterinary microbiology and pathobiology (Veterinary Bacteriology & my…irology, immunology, parasitology, pathology, and molecular biology).

Abstract

Peroxidase labeled immunoglobulins to camelpox virus (CPV) were prepared for use in various techniques of ELISA. Ten rabbits and three goats were inoculated with a mixture of camelpox virusand Freund's adjuvant. Sera were pooled separately on the 10th day post the last inoculation and immunoglobulins were precipitated using saturated ammonium sulphate. The globulins were 2.8 g/dl and 2.5 g/dl for rabbits and goats respectively and used for peroxidase conjugation. The peroxidase labeled immunoglobulins were titrated and evaluated using direct solid phase ELISA, double antibody sandwich ELISA and dot immunoblot ELISA. The prepared conjugates gave specific and clear positive reactions till the dilution of 2000 and 1500 for rabbits and goats immunoglobulins respectively. The prepared labeled immunoglobulins could be successfully used in detection of camel pox viral antigen of local virulent and standard vaccinal strain of the virus using various ELISA techniques.

DOI

10.21608/jvmr.2005.77992

Keywords

ELISA, detection, camelpox virus

Authors

First Name

E. A.

Last Name

Aboul Soud

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Magda, M.

Last Name

Sayed

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

A. A.

Last Name

Badawi

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

M. M.

Last Name

Taha

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Elham A.

Last Name

El-Ebiary

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

A. M.

Last Name

Daoud

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo, Egypt

Email

-

City

-

Orcid

-

Volume

15

Article Issue

2

Related Issue

11646

Issue Date

2005-03-01

Receive Date

2020-03-18

Publish Date

2005-03-01

Page Start

227

Page End

231

Print ISSN

2357-0512

Online ISSN

2357-0520

Link

https://jvmr.journals.ekb.eg/article_77992.html

Detail API

https://jvmr.journals.ekb.eg/service?article_code=77992

Order

37

Type

Original Article

Type Code

891

Publication Type

Journal

Publication Title

Journal of Veterinary Medical Research

Publication Link

https://jvmr.journals.ekb.eg/

MainTitle

Preparation of diagnostic ELISA kits for detection of camelpox virus

Details

Type

Article

Created At

22 Jan 2023