A penta-dog inactivated cell culture vaccine was prepared to protect dogs against canine distemper virus, canine parvovirus, canine adenovirus1, 2 and rabies virus. The potency of this vaccine was compared with that of single inactivated vaccines prepared against each disease, in different groups of susceptible dogs. It was found that the protective dose of penta-dog vaccine (2ml) including the protective amounts of the five viral proteins resulted in full protection of vaccinated dogs against the challenge with virulent strain of the used viruses showing no antagonizing effect between each other with and no adverse postvaccinal reaction. So, the prepared inactivated cell culture penta-dog vaccine is a safe and potent vaccine for dogs which resulted in saving time, cost, and effort stress factors on animals and providing good immune statues.