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77913

Polymerase chain reaction for differentiation of Pasteurella multocida isolates from turkeys in comparison to strains incorporating in fowl cholera vaccine

Article

Last updated: 22 Jan 2023

Subjects

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Tags

Veterinary microbiology and pathobiology (Veterinary Bacteriology & my…irology, immunology, parasitology, pathology, and molecular biology).

Abstract

In the present study, polymerase chain reaction (PCR) using random primer (E-20) was used to characterize and identify strains included in this study. Strains included 4 vaccinal reference strains of Pasteurella multocida, CU strain and 4 field isolates of Pasteurella multocida isolated from diseased turkeys which were identified biochemically and serologically as A:1, A:3, A3x4 and D:11. The obtained results revealed that all strains were reacted positively and in different manner with the E20 primer except the 2 field isolates. The results of these reactions demonstrated in terms of bands of different molecular weight specific to each strain. This can be used as a base for characterization and differentiation of strains involved in the present study as the 2 field strains A:1 and A:3 react with primer. Mouse protection test was performed by vaccination of mice with local fowl cholera oil adjuvant vaccine then challenge with virulent field strains A:1, A:3, D:12 and untypable isolates. Results revealed that the local fowl cholera adjuvant vaccine could protect mice against virulent challenge with A:1, A:3 and D:12 field strains but it could not be protect mice against untypable isolates

DOI

10.21608/jvmr.2007.77913

Keywords

Polymerase, chain, reaction, Pasteurella, multocida, isolates, turkeys, Strains, incorporating, vaccine, fowl cholera

Authors

First Name

Nahed I.M.M.

Last Name

Khamis

MiddleName

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Affiliation

Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.

Email

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City

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Orcid

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First Name

Zeinab M.

Last Name

Souror

MiddleName

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Affiliation

Central Laboratory for evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt.

Email

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City

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Orcid

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First Name

Hanan N.

Last Name

Ibrahim

MiddleName

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Affiliation

Central Laboratory for evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt.

Email

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City

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Orcid

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First Name

S. M.

Last Name

Aboul Saoud

MiddleName

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Affiliation

Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt. 2

Email

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City

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Orcid

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Volume

17

Article Issue

2

Related Issue

11642

Issue Date

2007-03-01

Receive Date

2020-03-18

Publish Date

2007-03-01

Page Start

21

Page End

24

Print ISSN

2357-0512

Online ISSN

2357-0520

Link

https://jvmr.journals.ekb.eg/article_77913.html

Detail API

https://jvmr.journals.ekb.eg/service?article_code=77913

Order

4

Type

Original Article

Type Code

891

Publication Type

Journal

Publication Title

Journal of Veterinary Medical Research

Publication Link

https://jvmr.journals.ekb.eg/

MainTitle

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Details

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Article

Created At

22 Jan 2023