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77892

Molecular characterization of Corynebacterium pseudotuberculosis isolated from sheep by random amplified polymorphic DNApolymerase chain reaction (RAPD-PCR)

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Veterinary clinical research (Veterinary Surgery, theriogenology, inte…seases, clinical pathology, applied epidemiology and animal hygiene).

Abstract

The phenogenetic and phylogenetic relationships among 7 identified strains of C.
pseudotuberculosis isolated from sheep were checked by PCR specific primers demonstrated with random amplified polymorphic DNA – polymerase chain reaction (RAPD – PCR) by using five arbitrarily primers. C pseudotuberculosis isolates were distinguished according to the banding patterns of their amplified DNA on agarose gel. The variation can be used for diagnostic differentiation among C.  pseudotuberculosis strains. Differences were observed in RAPD patterns between the 7 isolates and this may be due to the presence of novel serovars of this microorganism.

DOI

10.21608/jvmr.2007.77892

Keywords

Characterization, Molecular, Corynebacterium, pseudotuberculosis, isolated, sheep, random, amplified, polymorphic, DNA, Polymerase, RAPD-PCR

Authors

First Name

Azza S.A.

Last Name

Goda

MiddleName

-

Affiliation

Animal Health Department, Desert Research Center, Materia,Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

Mona A.

Last Name

Mahmoud

MiddleName

-

Affiliation

Animal Health Department, Desert Research Center, Materia,Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Wafaa A.

Last Name

Osman

MiddleName

-

Affiliation

Animal Health Department, Desert Research Center, Materia,Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

N.A.

Last Name

Ziada

MiddleName

-

Affiliation

Animal Health Department, Desert Research Center, Materia,Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

I. M.

Last Name

Moussa

MiddleName

-

Affiliation

Department. of Microbiology, Faculty of Veterinary Medicine, Cairo University, Gizza, Egypt

Email

-

City

-

Orcid

-

Volume

17

Article Issue

1

Related Issue

11641

Issue Date

2007-03-01

Receive Date

2020-03-18

Publish Date

2007-03-01

Page Start

39

Page End

49

Print ISSN

2357-0512

Online ISSN

2357-0520

Article File

JVMR_Volume 17_Issue 1_Pages 39-49.pdf

PDF . 3.6MB

Link

https://jvmr.journals.ekb.eg/article_77892.html

Detail API

https://jvmr.journals.ekb.eg/service?article_code=77892

Order

8

Type

Original Article

Type Code

891

Publication Type

Journal

Publication Title

Journal of Veterinary Medical Research

Publication Link

https://jvmr.journals.ekb.eg/

MainTitle

Molecular characterization of Corynebacterium pseudotuberculosis isolated from sheep by random amplified polymorphic DNApolymerase chain reaction (RAPD-PCR)

Details

Type

Article

Created At

22 Jan 2023