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77889

Molecular strategies for the differentiation and identification of local E. coli isolated from chicken: I. Characterization of protein profile.

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Veterinary microbiology and pathobiology (Veterinary Bacteriology & my…irology, immunology, parasitology, pathology, and molecular biology).

Abstract

In this study five serotypes of E. coli were isolated from chickens and identified as O1, O2, O6, O78 and O126 out of 33 isolates derived from a total of 60 samples. SDS-PAGE revealed that four proteins were characteristic and shared in all these serotypes at the molecular weight of 21, 30, 55 and 74 kDa of which 55 and 74 kDa proteins were fully reacted with the antisera against E. coli in the western blot. Other proteins are present but varied from one serotype to another.

DOI

10.21608/jvmr.2007.77889

Keywords

Molecular, strategies, differentiation, identification, local, E. coli, isolated, chicken, Characterization, protein, profile

Authors

First Name

S. S.

Last Name

Salama

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologices, Abbasia, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

Afaf A.

Last Name

Kheder

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologices, Abbasia, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

Elham A.

Last Name

Elebiary

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologices, Abbasia, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

M. M.

Last Name

Taha

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologices, Abbasia, Cairo, Egypt.

Email

-

City

-

Orcid

-

Volume

17

Article Issue

1

Related Issue

11641

Issue Date

2007-03-01

Receive Date

2020-03-18

Publish Date

2007-03-01

Page Start

25

Page End

28

Print ISSN

2357-0512

Online ISSN

2357-0520

Article File

JVMR_Volume 17_Issue 1_Pages 25-28.pdf

PDF . 235.2kB

Link

https://jvmr.journals.ekb.eg/article_77889.html

Detail API

https://jvmr.journals.ekb.eg/service?article_code=77889

Order

5

Type

Original Article

Type Code

891

Publication Type

Journal

Publication Title

Journal of Veterinary Medical Research

Publication Link

https://jvmr.journals.ekb.eg/

MainTitle

Molecular strategies for the differentiation and identification of local E. coli isolated from chicken: I. Characterization of protein profile.

Details

Type

Article

Created At

22 Jan 2023