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77831

Isolation and identification of bovine herpes virus -1 (BHV-1) from semen of foreign breeds bulls

Article

Last updated: 22 Jan 2023

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Subjects

-

Tags

Veterinary microbiology and pathobiology (Veterinary Bacteriology & my…irology, immunology, parasitology, pathology, and molecular biology).

Abstract

Bovine herpes virus-1 (BHV-1) was isolated from bull semen by inoculation onto chorioallantoic membrane of specific pathogen free eggs. The isolated virus was identified by agar gel precipitation test, Dot ELISA, pock reduction and neutralization test, as well as by histopathology. The isolated virus was propagated on Madin Darby Bovine Kidney cells and identified by polymerase chain reaction. In sero-survey for BHV-1 antibodies on 1091 collected serum samples 188 sera showed clear precipitation lines by AGPT

DOI

10.21608/jvmr.2008.77831

Keywords

isolation, identification, bovine herpes, virus, BHV-1, foreign, breeds, bulls

Authors

First Name

H. M.

Last Name

Madbouly

MiddleName

-

Affiliation

Virology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.

Email

-

City

-

Orcid

-

First Name

S. M.

Last Name

Tamam

MiddleName

-

Affiliation

Virology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.

Email

-

City

-

Orcid

-

First Name

A. M.

Last Name

Abd-El-Gaid

MiddleName

-

Affiliation

Virology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.

Email

-

City

-

Orcid

-

Volume

18

Article Issue

2

Related Issue

11636

Issue Date

2008-03-01

Receive Date

2020-03-17

Publish Date

2008-03-01

Page Start

22

Page End

27

Print ISSN

2357-0512

Online ISSN

2357-0520

Article File

JVMR_Volume 18_Issue 2_Pages 22-27.pdf

PDF . 237.3kB

Link

https://jvmr.journals.ekb.eg/article_77831.html

Detail API

https://jvmr.journals.ekb.eg/service?article_code=77831

Order

5

Type

Original Article

Type Code

891

Publication Type

Journal

Publication Title

Journal of Veterinary Medical Research

Publication Link

https://jvmr.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023