This study was carried out on twelve Holstein dairy cows. Five cows were clinically healthy and considered as control; three cows with left displacement of abomasum (LDA) and four cows with right displacement of abomasum (LDA). Diagnosis of DA based on clinical examinations and confirmed by ultrasonography. The Hb, PCV%, total leucocytic count, neutrophils and abomasal fluid pH were significantly increased (P<0.05) in RDA compared to control. Serum biochemical analysis showed significant reductions of sodium, potassium, chloride and calcium in LDA and RDA and significant increases (P < 0.05) in glucose and total proteins in RDA compared to the control. Moreover, there were significant increases (P<0.001) in ALT, AST, LDH, CPK, urea and creatinine in LDA and RDA compared to control. In conclusion, abomasal displacement produces alterations in the clinical, hematological and biochemical picture compared to control healthy cow and these alterations may vary according to the type of displacement