This study was carried out on serum samples collected from broiler breeder chicken flocks vaccinated with avian influenza (AI) H5N1 inactivated vaccine. These flocks included 23 flocks aged 13 to 47 weeks reared in close houses in 7 sites; two vaccinated breeder flocks for HI antibody monitoring by 5 weeks interval samples and 8 flocks aged 41 weeks reared in different sites with identified females and males samples. The vaccine was used in a dose of 0.2 ml at 1 day in hatchery and revaccinated with 0.5 ml at age of 18 days, 19- 20 weeks and 40 weeks. Hemagglutination inhibition (HI) test was carried out against homologous antigen. The study pointed out that AI H5N1 inactivated vaccine under field application induced irregular and low HI titres following the 1st two doses ranged from log 2 0.0 to 4.15 with great variation between flocks, where samples with titre 0-2 ranged from 20 to 100%. The 3rd dose at 19-20 weeks was essential to elevate HI titres 3.25 to 7.44 with more homogenizes flock immunity and lower percentage of titres 0-2 ( 0-20 %) and as measured by HI test. Revaccination of layer flocks at 40 weeks (fourth dose) improves flock immunity facing stress of egg production as evaluated by HI (5.52 - 6.33) and lower negative percentage (5.5-11.7%). Monitoring of breeder flock every 5 weeks is essential to detect proper time of revaccination as each flock has its HI antibody curve. There was a difference in HI tit re rang log 2 0.33 to 1.2 between male and female chicks reared in the same house, but this variation not affecting flock mean. Birds at aged 41 weeks having titres < log2 3 (Seronegative) were protected when exposed to contact with infected flock as showed no clinical signs or change in HI titres after 12 days. In conclusion the usage of homologous inactivated H5N1 vaccine in 4 doses in layer flocks was of value in improving chicken immunity to AI H5N1 wild strain circulate in our field