Twenty five isolates of IBV were isolated from 36 broiler and layer chicken farms collected from 13 governorates during 2 years started from January 2003. Sixteen farms were vaccinated against IB, and 9 farms were not vaccinated. The cardinal signs of the disease in layers were drop in egg production, with watery albumen, inferior (pale and misshape shell) eggs, un-noticed respiratory distress and pectoral myopathy, and those in broilers were respiratory distress, renal urate deposition and death beyond 4 weeks of age (late mortality). The viruses were isolated and identified by chicken embryo, and CEK cell culture inoculation.