A total of two hundreds of slaughtered animals; 50 each of cattle buffaloes, sheep and buffalo calves were collected from slaughterhouse Giza. Each animal was represented by muscular part, prenephric fat, liver, and kidneys. The Four Plate Technique (FPT) is intended to detect antimicrobial residues in collected samples. The liver samples showed high detection incidences (30, 16, 16 and 8%) in cattle, buffaloe, sheep and buffaloe calves respectively as compared to kidney samples (16, 12, 10 and 6% respectively). The lowest incidence was detected in fat samples (0%) followed by muscle samples (4%). High incidence was detected in medium I followed by medium II and III, finally medium IV as well as medium V which failed to be showed any incidence of detection. The tetracycline residues in tissues of slaughtered animals depend on husbandry as well as on withdrawal time after use. The liver (67 + 15, 50 + 14, 54 + 10 and 3 +0.8) and kidney (63 + 16, 46 + 12, 56 + 12 and 8 + 2) samples in cattle, buffaloe, sheep and buffaloe calves respectively showed high residual levels as compared with muscles and fat. The detection of sulfonamide residues in tissues of slaughtered animals may be attributed to misuse of these compounds for long term in animal feed as well as result in accumulation in animal tissues.