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77590

Genomic identification of Pasteurella multocida isolated from turkey flock in Egypt early 2008

Article

Last updated: 04 Jan 2025

Subjects

-

Tags

Veterinary clinical research (Veterinary Surgery, theriogenology, inteā€¦seases, clinical pathology, applied epidemiology and animal hygiene).

Abstract

Molecular detection and differentiation of Pasteurella multocida strain involved in a separate fowl cholera outbreak in a turkey flock farm located in El-Menofia Governorate, Egypt early 2008 was investigated. The isolated strain was compared with an Egyptian Pasteurella multocida isolate that was previously isolated from turkey flock during last decade besides four vaccinal strain (A:5, A:8, A:9 and D:2) on phenotypic and genotypic characterization basis. Phenotypically all the strains were similar as all the strains produce non haemolytic colonies on blood agar, and all the strains revealed similar biochemial behaviour. On the other hand, the genomic typing of all the stains using rep-PCR techniques [repetitive BOX elements, enterobacterial repetitive intergenic consensus (ERIC) and repetitive extragenic palindromic (REP) polymerase chain reaction (PCR)] differentiated the six Pasteurella multocida strains into six different profiles. The molecular identity between the Pasteurella multocida 2008 strain and the previously isolated strain was 76.6 % and were ranged from 65.2% to 79.2% with the 4 vaccinal strains. These results reported the continuous mutations of the field Pasteurella multocida strains among poultry flocks in Egypt indicating the urgent need for the frequent and continuous molecular epidemiological investigations of fowl cholera outbreaks in various poultry flocks to detect these new strains and update the fowl cholera vaccines.

DOI

10.21608/jvmr.2020.77590

Keywords

Genomic, identification, Pasteurella, multocida, isolated, turkey, flock, Egypt, 2008

Authors

First Name

S.A.

Last Name

Nassif

MiddleName

-

Affiliation

Central laboratory for evaluation of veterinary biologics

Email

-

City

-

Orcid

-

First Name

Hanan

Last Name

M. Ibrahim

MiddleName

-

Affiliation

Central laboratory for evaluation of veterinary biologics,Faculty of Agriculture, Ain-Shams University

Email

-

City

-

Orcid

-

First Name

Zeinab

Last Name

M. Souror

MiddleName

-

Affiliation

Central laboratory for evaluation of veterinary biologics,Faculty of Agriculture, Ain-Shams University

Email

-

City

-

Orcid

-

First Name

Arwa H.

Last Name

Elnaggar

MiddleName

-

Affiliation

Central laboratory for evaluation of veterinary biologics,Faculty of Agriculture, Ain-Shams University

Email

-

City

-

Orcid

-

First Name

Hayam

Last Name

Farouk

MiddleName

-

Affiliation

Central laboratory for evaluation of veterinary biologics,Faculty of Agriculture, Ain-Shams University

Email

-

City

-

Orcid

-

First Name

A. B.

Last Name

Abd Elrazek

MiddleName

-

Affiliation

Central laboratory for evaluation of veterinary biologics,Faculty of Agriculture, Ain-Shams University

Email

-

City

-

Orcid

-

First Name

M. M.

Last Name

Yousef

MiddleName

-

Affiliation

Central laboratory for evaluation of veterinary biologics,Faculty of Agriculture, Ain-Shams University

Email

-

City

-

Orcid

-

First Name

Elham.A.

Last Name

Elebiary

MiddleName

-

Affiliation

Central laboratory for evaluation of veterinary biologics,Faculty of Agriculture, Ain-Shams University

Email

-

City

-

Orcid

-

Volume

20

Article Issue

1

Related Issue

11613

Issue Date

2010-03-01

Receive Date

2020-03-16

Publish Date

2010-03-01

Page Start

134

Page End

141

Print ISSN

2357-0512

Online ISSN

2357-0520

Link

https://jvmr.journals.ekb.eg/article_77590.html

Detail API

https://jvmr.journals.ekb.eg/service?article_code=77590

Order

18

Publication Type

Journal

Publication Title

Journal of Veterinary Medical Research

Publication Link

https://jvmr.journals.ekb.eg/

MainTitle

Genomic identification of Pasteurella multocida isolated from turkey flock in Egypt early 2008

Details

Type

Article

Created At

22 Jan 2023