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77694

Sero-diagnosis of Bovine Tuberculosis by ELISA Using Bovine PPD and ST.CF

Article

Last updated: 22 Jan 2023

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Subjects

-

Tags

Veterinary clinical research (Veterinary Surgery, theriogenology, inte…seases, clinical pathology, applied epidemiology and animal hygiene).

Abstract

Bovine tuberculosis represents one of the very important infectious diseases in Egypt and the  world. It has zoonotic importance and causes severe economic losses. Accurate and rapid diagnosis considered as the milestone for control of the disease. In this study ELISA technique was used for confirmation of positive reactors cows that tested with single intradermal tuberculin test, to detect false positive reactors. Bovine PPD and ST.CF antigens have been used as two different coating
antigens for ELISA technique. 3747 cattle from dairy farms in five different governorates were subjected to the single intradermal cervical tuberculin test whereas 78 (2.24%) proved positive reactors to tuberculin. These positive reactors tested with ELISA. 64 (82.05%) animals were positive by ELISA coated with ST-CF, while by using bovine PPD as coating antigen 58 (74.35%) animals were positive. The previous results indicated that ELISA test showed higher sensitivity and specificity using ST-CF as coating antigen than in case of bovine PPD coating antigen. 

DOI

10.21608/jvmr.2013.77694

Keywords

Sero-diagnosis, bovine, tuberculosis, ELISA, PPD, ST.CF

Authors

First Name

A.

Last Name

El-Sify

MiddleName

-

Affiliation

Department of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Sadat City Branch, Minufyia Univeristy, Egypt

Email

-

City

-

Orcid

-

First Name

M.

Last Name

Nayel

MiddleName

-

Affiliation

Department of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Sadat City Branch, Minufyia Univeristy, Egypt

Email

-

City

-

Orcid

-

First Name

S.

Last Name

Hazem

MiddleName

-

Affiliation

Departement of Immunology and Immunopharmacolgy, Animal Reproduction Research Institution, Egypt

Email

-

City

-

Orcid

-

First Name

R.

Last Name

Tarabess

MiddleName

-

Affiliation

Department of Bacteriology, Mycology and Immunology Faculty of Veterinary Medicine, Sadat City Branch, Minufyia Univeristy, Egypt

Email

-

City

-

Orcid

-

First Name

S.

Last Name

Akram

MiddleName

-

Affiliation

Department of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Sadat City Branch, Minufyia Univeristy, Egypt

Email

-

City

-

Orcid

-

First Name

M.

Last Name

Allaam

MiddleName

-

Affiliation

Department of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Sadat City Branch, Minufyia Univeristy, Egypt

Email

-

City

-

Orcid

-

First Name

H.

Last Name

Hassan

MiddleName

-

Affiliation

Department of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Sadat City Branch, Minufyia Univeristy, Egypt

Email

-

City

-

Orcid

-

First Name

M.

Last Name

El Garhy

MiddleName

-

Affiliation

Agriculture Extension Sector, Ministry of Agriculture, Egypt

Email

-

City

-

Orcid

-

Volume

22

Article Issue

1

Related Issue

11611

Issue Date

2013-03-01

Receive Date

2020-03-17

Publish Date

2013-03-01

Page Start

126

Page End

129

Print ISSN

2357-0512

Online ISSN

2357-0520

Article File

JVMR_Volume 22_Issue 1_Pages 126-129.pdf

PDF . 160.7kB

Link

https://jvmr.journals.ekb.eg/article_77694.html

Detail API

https://jvmr.journals.ekb.eg/service?article_code=77694

Order

20

Type

Original Article

Type Code

891

Publication Type

Journal

Publication Title

Journal of Veterinary Medical Research

Publication Link

https://jvmr.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023