Buxtonella sulcata (Jameson, 1926) is an intestinal protozoan of large ruminants, with scanty information and contradictious reports about its pathogenesity. This work aimed to investigate the prevalence rate of B. sulcata in Egypt. Forty eight cases collected from cattle (n= 29) and buffaloes (n= 19) from El-Mahalla El-Kubra area, Al-Gharbiya province. Samples were examined for the presence B. sulcata. The overall infection rate was 41.6% (20/48), in cattle 48.2% (14/29) and in buffalo 31.5% (6/19). This is the first study on B. sulcata in Egyptian ruminants also highlights the situation of intestinal ciliates of ruminant animals and provides basic information for the future work of intestinal ciliates of animals and man