In this study multiplex PCR was used for typing of Clostridium perfringens isolates from soil, clinically healthy and diseased sheep. Clostridium perfringens was isolated from 41 out of 100 soil samples, 12 out of 100 clinically healthy sheep and 118 out of 200 sheep with enterotoxaemia signs. Genotyping of 41 isolates from soil indicated that 29 (70.73%) were type A, 3 (7.31%) were type B and 9 (21.95%) were type D. Of 12 isolates from clinically healthy sheep 6 (50%) were type A and 6
(50%) were type D. Of 118 isolates from diseased sheep 42 (35.59%) were type A, 22 (18.64%) were type B and 54 (45.76%) were type D. This result indicates that Clostridium perfringens type A, B and D are the main types causing enterotoxaemia in sheep in Egypt and Clostridium perfringens type A must be included in any vaccine programme to ensure optimum protection.