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77665

Staphylococcus aureus protein "A" as a rapid and sensitive test in comparative with other serological tests for detection of Rift Valley Fever antibodies in vaccinated camels

Article

Last updated: 22 Jan 2023

Subjects

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Tags

Veterinary microbiology and pathobiology (Veterinary Bacteriology & my…irology, immunology, parasitology, pathology, and molecular biology).

Abstract

Rift Valley Fever (RVF) is a viral zoonotic disease transmitted by mosquitoes leading to abortion and death mainly in livestock. The present study evolved serum samples obtained from 100 RFV vaccinated as well as 10 non vaccinated camels tested for RVF antibodies using different serological techniques. Freshly prepared Staphylococcus aureus protein "A" (SPA) was used as a rapid, sensitive, specific and cheap screening test which did not need specific or expensive equipment. Also uses of SPA-ELISA test without need antispecies conjugate, SNT and AGPT. Inactivated RVF virus was used in all serological tests except SNT. The results showed that Staphylococcus aureus Protein Acoagglutination test (SPA-COAT) was 78%, SPA-ELISA 82%, SNT 75% and AGPT 30%. The degree of positive samples with (++++) and (+++) of SPA-COAT were also positive in SPA-ELISA and SNT, but samples with (++++) SPA-COAT were only positive in AGPT. We could recommend the use of SPA-COAT, as a universal technique, in wide range of veterinary medicine as an effective and economic method which acts a non species specific for serological screening field test as well as a laboratory test

DOI

10.21608/jvmr.2011.77665

Keywords

Staphylococcus, rapid, Comparative, serological, camels

Authors

First Name

M.A.M.

Last Name

Saad

MiddleName

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Affiliation

Animal Reproduction Research Institute, Al-Haram, Giza

Email

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City

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Orcid

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First Name

Hala,

Last Name

A. Salem

MiddleName

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Affiliation

Animal Health Research Institute, Dokki, Giza.

Email

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City

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Orcid

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First Name

A. A.

Last Name

Ibrahim

MiddleName

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Affiliation

Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo.

Email

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City

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Orcid

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Volume

21

Article Issue

1

Related Issue

11608

Issue Date

2011-03-01

Receive Date

2020-03-17

Publish Date

2011-03-01

Page Start

4

Page End

5

Print ISSN

2357-0512

Online ISSN

2357-0520

Link

https://jvmr.journals.ekb.eg/article_77665.html

Detail API

https://jvmr.journals.ekb.eg/service?article_code=77665

Order

11

Type

Original Article

Type Code

891

Publication Type

Journal

Publication Title

Journal of Veterinary Medical Research

Publication Link

https://jvmr.journals.ekb.eg/

MainTitle

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Details

Type

Article

Created At

22 Jan 2023