A total of 225 samples were collected from suspected cases and they were 75 human samples (40 ear swabs, 29 vaginal swabs and 6 sputum samples) and 150 from broiler chickens for microbiological examination and fungal isolation, they collected from different areas in El-Fayoum and BeniSuef governorates, from which 129 fungal isolates were recovered, 22 fungal isolates( 29.3%) were recovered from human; of which 15 isolates of ear swabs ( A.fumigatus 37.5%) while 7 (24.1%) A.fumigatus isolates were recovered from women, but there was no A.fumigatus recovered from sputum samples, as well as there were 53 A.fumigatus isolates (35.3%) recovered from broiler chicken. The antifungal activities of thymol and carvacrol oils against the recovered fungal isolates were tested using agar dilution method. Thymol and carvacrol oils completely inhibited the growth of different fungal isolates at concentrations of 1% and 0.1%. On the other hand, the concentration of 0.01% was too weak to inhibit the fungal growth, but it completely reduced the colour of the fungal colony converted it into white coloured arial mycelium. PCR assay using oligonucleotide primer that amplifying 250bp fragment in its Gene of A.fumigatus and A.niger was performed. Sequence analysis of two isolates of A.niger and A.fumigatus using its Gene was performed.