This study aimed at investigating the physicochemical and bacteriological status of retail-marketed crustaceans in Beni-Suef Governorate, Egypt. Therefore, 120 samples of crustaceans, 60 samples of imported unpeeled shrimp, and 60 samples of locally harvested chilled crab were collected from different fish markets in Beni-Suef Governorate. Collected samples were examined through assessing the physicochemical deterioration criteria in terms of pH and total volatile basic nitrogen (TVB-N), and the bacterial load including mesophilic count, psychrophilic count, Staphylococcus (Staph.) aureus count and most probable number (MPN) of coliforms, faecal coliforms and E. coli. As well as isolation and biochemical identification of pathogenic E. coli and Staph. aureus. The obtained results revealed that high percentages of examined crustaceans had pathogenic E. coli and Staph. aureus, however, total mesophilic, psychrophilic and Staph. aureus counts in most examined crustacean samples lie within the recommended permissible limits by national and international organizations. Similarly, pH and TVB-N mean valwere within acceptable limits. We conclude that crustaceans marketed in Beni-Suef are subjected to inadequate hygienic measures during processing, time/temperature abuse, inappropriate handling and unsatisfactory personnel hygiene.