lumpy skin disease virus (LSDV) was isolated, from naturally infected cattle that have a history of previous vaccination with live attenuated sheep pox virus (SPV) vaccine. The virus was isolated on chorio-allantoic membranes (CAM) of embryonated chicken eggs (ECE) and Madin Darby Bovine Kidney Cells (MDBK) and identified by agar gel precipitation test (AGPT) and immunofluorescent antibody technique (IFA). Characteristic pock lesions and intracyptoplasmic flourescene granules are identified respectively. Molecular characterization using polymerase chain reaction (PCR) using specific primer for G-Protein Coupled Chemokine Receptor Gene of LSDV isolates specific amplified product 554 bp. Sequence analysis revealed tow new isolates of LSDV.