The present study aimed to prepare a combined inactivated vaccine containing
bovine viral diarrhea genotype-1(BVD-1),bovine viral diarrhea genotype-2 (BVD-
2),infectious bovine rhinotracheitis (IBR),parainfluenza-3 (PI-3) and bovine
respiratory syncytial virus(BRSV) and adjuvanted with montanide oil ISA 206.
Quality control results proved that the pneumo-5 vaccine was pure and completely
safe to be used in calves without abnormalities. Potency test was performed on
two groups of calves three for each group, where the first group was vaccinated
with pneumo-5 vaccine adjuvant with montanide oil ISA 206 and the second group < br />was left as non-vaccinated control group. In group (1), serum neutralization test
revealed that the serum neutralizing antibody titers in BVD-1 and BVD-2 developed
more higher than the minimal acceptable titer of the protective level (log10 0.9),
while log10 0.6 was protective against IBR, PI-3 and BRS viruses at one month of
vaccination and remained protective till the end of experiment compared to group < br />(2) that showed no neutralizing antibody response. The prepared vaccine proved
to be highly potent as the developed BVD-1, BVD-2, IBR, PI-3 and BRSV antibodies
remained within the protective level for 9 months post vaccination.