Bacteria could adhere and invade various tissues result in diverse pathologic lesions in concordance to the localization site. Proventriculitis reduces growth rate ends with huge economic losses. Microbiological investigation of 99 proventriculitis specimens revealed the recovery of diverse bacterial species of clinical impact on poultry industry. As far as we know, P. aeruginosa was isolated as a first record and with the highest prevalence amongst the recovered bacterial species (39.4%). C. perfringens and members of Enterobacteriaceae (P. mirabilis, Citrobacter spp., E. coli, E. aerogenes and K. pneumoniae) were isolated with variable prevalence. E. coli represented 8.1% of the overall bacterial species isolated and they were serogrouped in E. coli O158 (75%) and O146 (25%). Regarding antimicrobial resistance 100% of the examined P. aeruginosa and E. coli isolates were multidrug resistance. Extended Spectrum Beta-Lactamase (ESBLs) and AmpC detected in P. aeruginosa with 12.8 and 97.9% respectively and in E. coli they were detected in 37.5 and 12.5% respectively. The current study indicates that the bacterial proventriculitis not only influences broilers economy but also could threaten human health via bacterial species of zoonotic potential and probability of transferring their antimicrobial resistance determinants.