> Bovine ephemeral fever (BEF) is an acute, arthropod-borne disease of cattle. The disease is
characterized by sudden onset of fever, high morbidity and very low mortality. Recovery
occurs within three days of the onset of clinical signs. BEF is an important viral disease of
cattle in Egypt so the live attenuated BEFV vaccine which is inactivated just before
inoculation by reconstitute in PBS containing saponin. is extensive used for the prevention
and control of the disease. Different assays were applied in the current study to quality
control evaluate of that produce vaccine by detection of viral identity and viability before
and after reconstitution by using real time quantitative reserve transcriptase polymerase
chain reaction (qRT-PCR) and clinical findings (Body temperature and clinical signs) and
potency by measuring the humoral immune response by serum neutralization test (SNT) and
ELISA and cellular immune response by interferon gamma (IFN-γ) using ELISA kit and
Quantitative real time polymerase chain reaction (qRT-PCR) and also by lymphocyte cell
proliferation assay using tetrazolium salt(XTT).