Embryo's quality is closely linked to miRNA regulation, which plays a critical role in gene expression and protein synthesis. In this prospective cohort observational study, extracellular miRNAs were tested using the miRCURY Locked Nucleic Acid (LNA) system, which used culture media from twenty-two singly transferred embryos. Study samples were collected on day three and embryos were transferred on day five. Based on earlier publications, hsa-miR-29c-3p and hsa-miR-30c-5p, among other miRNAs (not published), were chosen and assessed. The negative-implantation group had higher expression of hsa-miR-30c-5p regarded as statistically significant (P.value <0.001) than the other tested miRNAs. Contrary, there was no significant link between pregnancy outcome and expressed hsa-miR-29c-3p. Study findings, in comparison with findings from other reports, reveal the absence of hsa-miR-29c-3p and hsa-miR30c-5p from day 3 implanting embryos in contrast with day 5 where they are abundant, suggesting specificity to the embryo's development from cleavage to morula, blastocyst, and implantation stages. These findings could open the gate toward potential biomarkers for embryo development and implantation outcome.