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269398

Evaluating New Biomarkers for Epstein Barr Virus Infection in Egypt

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Last updated: 04 Jan 2025

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Abstract

Some reports have suggested EBV to be a trigger agent for an autoimmune hepatitis, even EBV has been suspected as a probable cause of particular granulomas in the liver and even in a rare vanishing bile duct syndrome. This study aimed to evaluate CD19+, CD23+ and CD24+ B- lymphocytes as biomarkers for EBV early diagnosis in EBV-liver disease patients. Subjects & Fifty subjects included in this work, grouped into: control group and EBV mono-infected patients' group.  Immunophenotyping in peripheral blood samples was performed for CD19+, CD23+ and CD24+ B-cells using flow cytometry technique. CD23+ B-cells frequency was the most significant (P <0.001) changed CD marker- among the estimated CDs- in EBV group versus control group (49.43±0.58 vs 21.18±1.10), respectively. Both CD19+ and CD23+ B-cells frequency correlate strongly with liver transaminases.  Diagnostic performance of this panel of CD markers was obtained by applying receiver operating characteristic (ROC) curve analysis; CD23+ B-cell frequency was the most precise CD marker- of this panel- in distinguishing EBV cases from controls. The AUC of CD23+ B-cell frequency was 0.998, with sensitivity= 96% and specificity= 100% at cut-off value of 44.4%. Its NPV was ultimate (100%). On the other hand, CD19+ B-cells frequency did not show any response as diagnostic tool, and CD24+ B-cells frequency showed very little response in this context.  CD23+ B-cell frequency has a strong correlation with liver transaminases which may mirrored the severity of the disease. Additionally, it may represent a promising co-biomarker with the currently used EBV- viral capsid antigen (EBV-VCA) antibodies for early EBV diagnosis. 

DOI

10.21608/rjab.2019.269398

Keywords

<i>EBV, early diagnosis, CD19+, CD23+, CD24+, Flow cytometry</i>

Authors

First Name

Hamdy

Last Name

Keshk

MiddleName

E.

Affiliation

El-Azhar University, Cairo, Egypt

Email

hamde.elsayed@yahoo.com

City

-

Orcid

-

First Name

Randa

Last Name

Talaat

MiddleName

M.

Affiliation

Molecular Diagnostics and Therapeutics Department, Genetic Engineering & Biotechnology Research Institute (GEBRI), University of Sadat City (USC), Elsadat city, Egypt.

Email

randa.mohamed@gebri.usc.edu.eg

City

-

Orcid

-

First Name

Osama

Last Name

Shalaan

MiddleName

F.

Affiliation

Molecular Diagnostics and Therapeutics Department, Genetic Engineering & Biotechnology Research Institute (GEBRI), University of Sadat City (USC), Elsadat city, Egypt.

Email

-

City

-

Orcid

-

First Name

Mofida

Last Name

keshk

MiddleName

A.

Affiliation

Molecular diagnostics and therapeutics department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt.

Email

mofida.keshk@gebri.usc.edu.eg

City

-

Orcid

-

Volume

5

Article Issue

2

Related Issue

31330

Issue Date

2019-12-01

Receive Date

2019-11-26

Publish Date

2019-12-01

Page Start

22

Page End

31

Print ISSN

2356-9433

Online ISSN

2682-3470

Article File

RJAB_Volume 5_Issue 2_Pages 22-31.pdf

PDF . 411.7kB

Link

https://rjab.journals.ekb.eg/article_269398.html

Detail API

https://rjab.journals.ekb.eg/service?article_code=269398

Order

2

Type

Original Article

Type Code

890

Publication Type

Journal

Publication Title

Research Journal of Applied Biotechnology

Publication Link

https://rjab.journals.ekb.eg/

MainTitle

Evaluating New Biomarkers for Epstein Barr Virus Infection in Egypt

Details

Type

Article

Created At

22 Jan 2023