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106784

Genetic variations among two Egyptian mint species (Mentha spicata and Mentha piperita) using random amplified Polymorphic DNA markers

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Last updated: 22 Jan 2023

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Abstract

In the present study, RAPD-PCR was used for identification of two mint species (Mentha spicata and Mentha piperita) collected from Egypt and studying the genetic variations among them. RAPD-PCR was conducted using ten oligonucleotide primers. The ten primers succeeded in amplifying of DNA fragments for the two Mentha species. These primers produced multiple fragments profiles ranging from 3 to 19 fragments. The polymorphism levels differed from primer to another. The number of polymorphic fragments ranged from 0 to 66.29 per primer. The total number of the obtained fragments from the ten primers was 83 fragments with 39 polymorphic fragments (46.98 %) across the two mint species. Forty percent of primers did not exhibit any polymorphism. This study provides evidence that RAPD Markers can be used as an efficient tool for the detection of the genetic variations and identifications of different mint species.

DOI

10.21608/rjab.2018.106784

Keywords

Mentha species, RAPD-PCR, Genetic differences, molecular markers

Authors

First Name

Medhat

Last Name

Hashem

MiddleName

H.

Affiliation

Animal Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City, Egypt

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City

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Orcid

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First Name

Islam

Last Name

Rizk

MiddleName

M. H.

Affiliation

Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute(GEBRI), University of Sadat city, Sadat city, Egypt

Email

islam_rizk2@yahoo.com

City

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Orcid

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First Name

Reham

Last Name

Abd El-Azeem

MiddleName

M.

Affiliation

Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City, Egypt

Email

reham.abdelazeem@gebri.usc.edu.eg

City

-

Orcid

-

Volume

4

Article Issue

2

Related Issue

16177

Issue Date

2018-11-01

Receive Date

2020-08-09

Publish Date

2018-11-01

Page Start

1

Page End

8

Print ISSN

2356-9433

Online ISSN

2682-3470

Link

https://rjab.journals.ekb.eg/article_106784.html

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https://rjab.journals.ekb.eg/service?article_code=106784

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Type

Original Article

Type Code

890

Publication Type

Journal

Publication Title

Research Journal of Applied Biotechnology

Publication Link

https://rjab.journals.ekb.eg/

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Article

Created At

22 Jan 2023