A total of 50 albino rats were used to carry out an in vivo study on the cholesterol lowering impact of isolated L. acidophilus isolated from diatery supplements. Blood serum of the experimental rats as well as their liver, heart and aorta tissues were analyzed and examined .The attained results revealed a great decrease in serum cholesterol due to the use of live or dead bacterial cells. The histopathological results collected from examination of liver revealed that in live lactobacillus treated group display significant amelioration compare to non treated group, while no significant improvement detected in dead lactobacillus treated group. The corresponding results of the heart in showed that some pathological lesions seen in cardiac tissue in high cholesterol feeding group, still observed. This is besides atrophy of many cardiomyocytes and apoptosis. Signe of improvement was noticed in dead lactobacillus treated hypercholesterolemic group, where the severity and incidence of the pathological lesions observed in hypercholesterolemia animals was significantly reduced. Concerning the aorta, the attained results demonstrated that in live lactobacillus treated group, early plague formation was observed in few areas, however fibrin covered wide area of the endothelial layer. Significant amelioration in aorta tissues of dead lactobacillus treated hypercholesterolemia group was detected.