Silver nanoparticles play a vital role in the development of new antimicrobial substances against a number of pathogenic microorganisms. These nanoparticles due to their smaller size could be very effective as they can improve the antibacterial activity through lysis of bacterial cell wall. The biosynthesis of nanoparticles as an emerging highlight of the intersection of nanotechnology and biotechnology has received increasing attention due to a growing need to develop environmentally benign technologies in material synthesis (Kalishwaralal et al., 2008). In the present investigation, synthesis of silver nanoparticles (AgNPs) was attempted using the isolated pharmaceutical contaminants. The synthesized AgNPs were evaluated for their antibacterial activity against common pharmaceutical contaminants. When AgNPs were mixed with hydrogen peroxide disinfectant they displayed strong synergistic antibacterial. The overall results highlighted the potential use of commonly isolated microorganisms from pharmaceutical industrial areas in the synthesis of AgNPs and their utilization in various applications particularly as antibacterial substance in disinfection and preservation to protect against various biomedical, pharmaceutical based activities.