Non-alcoholic fatty liver disease (NAFLD), hepatic manifestation of metabolic syndrome, has been considered the most common liver disease nowadays. The aim of this study is to evaluate the potential therapeutic effect of pomegranate peel extract on the experimental rat model of non-alcoholic fatty liver diseases (NAFLD) and to explore the role of mir-33a and mir-34a as the molecular targets for these therapeutic effects.Fifty male albino Wistarrats were used in the present study. The NAFLD was induces in rats using high fat diet (HFD). The pomegranate peel extract (PPE) was used in different doses (50,100 and 150) for 30 days.The present study documents the ameliorating effects of PPE on hyperglycemia, insulin resistance, lipid profile and liver functions in NAFL rats in a dose-dependent manner compared to untreated rats.The ameliorating effects on the metabolic parameter are associated with significant correction in the hepatic expression of MiR-33a and MiR-34ain a dose-dependent manner. From the present study we can concluded that the PPE have efficient anti-steatotic effect which may be mediated through modulation of MiR-33a and MiR-34.