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57586

BIOREMEDIATION OF SOME AGRICULTURAL WASTES BY BACILLUS THURINGIENSIS FOR METALLOPROTEASE PRODUCTION

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Last updated: 04 Jan 2025

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Abstract

Agricultural wastes are very rich in nutrients that can be used as complete balanced microbiological media for growing microbes in order to produce valuable commercial products. In this study, a biodegradation process of wheat bran and rice bran wastes was carried out to produce metalloprotease enzyme from B. thuringiensis 1257strain which is considered to be a hyper producer for that enzyme. Metalloprotease was partially purified by ammonium sulfate followed by application on Sephadex G-75 column. Gel filtration step resulted in more than 40 times fold purification of the purified enzyme. The enzyme activity was inhibited by EDTA (almost 80%) at 15 mM concentration. These agro-industrial wastes were used as substrates for economic production of the enzyme from B. thuringiensis compared with some bacterial isolates collected from different sites in Kafr El-Sheikh and Menofiya governorate, Egypt. Optimum temperature for enzyme activity was 40 °C. It also exhibited a broad pH activity range (6-9) with an optimum pH of 8.

DOI

10.21608/rjab.2017.57586

Keywords

metalloprotease, B. thuringiensis1257, EDTA, agro-industrial waste, Sephadex G-75

Authors

First Name

Nashwa

Last Name

Rizk

MiddleName

M. H.

Affiliation

Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat city, Egypt

Email

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City

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Orcid

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First Name

Ayman

Last Name

Eldourghamy

MiddleName

S.

Affiliation

Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat city, Egypt

Email

eldorghamy@yahoo.com

City

-

Orcid

-

First Name

Eman

Last Name

Elgamal

MiddleName

G.

Affiliation

Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat city, Egypt

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City

-

Orcid

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Volume

3

Article Issue

1

Related Issue

7686

Issue Date

2017-06-01

Receive Date

2019-11-08

Publish Date

2017-06-01

Page Start

11

Page End

22

Print ISSN

2356-9433

Online ISSN

2682-3470

Article File

RJAB_Volume 3_Issue 1_Pages 11-22.pdf

PDF . 330.1kB

Link

https://rjab.journals.ekb.eg/article_57586.html

Detail API

https://rjab.journals.ekb.eg/service?article_code=57586

Order

9

Type

Original Article

Type Code

890

Publication Type

Journal

Publication Title

Research Journal of Applied Biotechnology

Publication Link

https://rjab.journals.ekb.eg/

MainTitle

BIOREMEDIATION OF SOME AGRICULTURAL WASTES BY BACILLUS THURINGIENSIS FOR METALLOPROTEASE PRODUCTION

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Article

Created At

22 Jan 2023