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Agricultural wastes are very rich in nutrients that can be used as complete balanced microbiological media for growing microbes in order to produce valuable commercial products. In this study, a biodegradation process of wheat bran and rice bran wastes was carried out to produce metalloprotease enzyme from B. thuringiensis 1257strain which is considered to be a hyper producer for that enzyme. Metalloprotease was partially purified by ammonium sulfate followed by application on Sephadex G-75 column. Gel filtration step resulted in more than 40 times fold purification of the purified enzyme. The enzyme activity was inhibited by EDTA (almost 80%) at 15 mM concentration. These agro-industrial wastes were used as substrates for economic production of the enzyme from B. thuringiensis compared with some bacterial isolates collected from different sites in Kafr El-Sheikh and Menofiya governorate, Egypt. Optimum temperature for enzyme activity was 40 °C. It also exhibited a broad pH activity range (6-9) with an optimum pH of 8.
DOI
10.21608/rjab.2017.57586
Keywords
metalloprotease, B. thuringiensis1257, EDTA, agro-industrial waste, Sephadex G-75
Authors
Affiliation
Environmental Biotechnology Department, Genetic Engineering and Biotechnology
Research Institute, University of Sadat City, Sadat city, Egypt
Email
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Environmental Biotechnology Department, Genetic Engineering and Biotechnology
Research Institute, University of Sadat City, Sadat city, Egypt
Email
eldorghamy@yahoo.com
City
-Orcid
-Affiliation
Environmental Biotechnology Department, Genetic Engineering and Biotechnology
Research Institute, University of Sadat City, Sadat city, Egypt
Email
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https://rjab.journals.ekb.eg/article_57586.html
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https://rjab.journals.ekb.eg/service?article_code=57586
Publication Title
Research Journal of Applied Biotechnology
Publication Link
https://rjab.journals.ekb.eg/
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