Some physical and chemical effects of cellulase on soils (sandy, calcareous and clay soil ) are investigated in this study. Pots filled with 5 kg soil were treated with Novozym 188 cellulase prepared (10 % solution ) at 0.5 and 1.5 % addition rates .Treatments resulted increasing of organic matter ( OM ), total and available nitrogen, cation exchange capacity ( CEC ) and total porosity ( TP ) of the studied soils. The soil properties increased with increasing the application rate of enzyme. Hydraulic conductivity (HC) was increased in clay and calcareous soils but, it was decreased in sandy soil . Electric conductivity ( EC ) and pH values were not affected by the enzyme addition .