It is well known that large percentages of nitrogen in the form of nitrate anions leach through soil especially when excessive amounts are applied to soil. Plant intake of nitrate is not fast enough to recover all the excessive amounts of nitrates from plants root zone. The relative slow utilization of nitrate by plants allow losses of nitrates by leaching out the root zone. On the other hand, ammonium cation is adsorbed by soil colloids, so it does not leach out of soil . Nitrification of NH⁺₄ to NO^-₃ in soil is also a source for nitrate . The aim of the present investigation was to look for how to reduce this risk and at the same time increase the economic reflect of the nitrogen unit in the soil by using organic manure treatment (town refuses [TR] , farmyard manure [FYM]), and biofertilizer (Nitrobin). Two pot experiments were conducted during the successive growing seasons of 1996/97 and 1997/98 at El-Serw Agriculture Station - Damietta. Each pot was filled with 15 kg of a sandy soil obtained from El-Rakabia. The experiment were designed as split-split plot , two treatments of microbial inoculation (I₀ and I₁) assigned the main plots. Five forms and levels of organic manure treatments (Control, 5g FYM, 10g FYM, 5g TR and 10g TR / kg soil) occupied the sub-plots, while, the nitrogen fertilizer levels (N₀, N₂₀, N₄₀, and N₈₀ mg N / kg soil) occupied the sub-sub plots . The total number of this trial were 40 treatments. Each treatment was replicated five times, hence 200 containers were conducted. The obtained results could be summarized as follows:-      Biofertilization of wheat grains by Nitroben and organic manure additions caused significant increases in the mean values of wheat dry weight (g/plant) during both seasons.      Significant decreases in leached NO^-₃ and NH⁺₄-N in drainage water were recorded due to biofertilization and manure application.      Nitrogen uptake high significantly increased due to both Nitrobin inoculation and manure treatments.      The highest means of utilization rate (UR) of applied nitrogen were produced due to organic manure additions.      Increasing the applied-N fertilizer tended to reduce the N-utilized percent. The obtained results illustrate the beneficial effects of bio and organic fertilization on reducing the adverse environmental impacts of inorganic nitrogen in agro-ecosystem.