Aeroponically-produced mycorrhizal fungus Glomus sp. was compared for growth-promoting potential, with two traditional sources of mycorrhizal nocula, namely soil-based pot culture of Glomus sp. and naturally-mixed AM fungi, in a pot experiment using maize and sorghum as host plants, under green house conditions. Four weeks after sowing, only plants which received infected root segments inocula gave moderate increases in plant growth parameters, compared with non-inoculated ones. However, spores inoculum produced non-significant differences in growth parameters of host plants. Eight weeks after sowing, soil-based pot culture treatment produced more root colonization ratios, shoot dry weights and phosphorus contents than those achieved with aeroponic culture treatment, being most pronounced with plants inoculated with infected root segments. At the final harvest, aeroponic culture of Glomus sp. produced root colonization ratios and plant-growth improvements similar to that achieved with soil-based pot culture treatment. However, (naturally mixed AM fungi) treatment gave the least increases in these parameters. These results indicated that, no biological differences were found  between the inoculum obtained from aeroponic culture and this collected from soil-based pot culture.