This study was conducted in an attempt to study the antimutagenic effect of volatile oil of Sa/via fruticosa plant in HGPRT gene of the fungus Coprince cinereus. On wet weight basis, the leaves of the plant contained 0.7% volatile oil which was yellow in color and had aromatic odour. The oil was characterized using TLC and GC-MS. The latter showed the presence of twenty three components, ten of them were found in high percent (1 ,8-Cineole • 1,8-Cineol Isomer - Carnphor. Trans-f-Caryophyllene, a-Pinene • ~-Myrcene. unalot. a-Terpineol. a-Terpineol isomer). Five different concentration were tested for their antimutagenic effect against the cyclophosphamid (CP) drug at the mutagenic dose (7~g/ml). The results indicating that the volatile oil was ineffective in preventing the toxic effect of the mutagenic drug. Further more, the tow treatment (before and after mutagenic drug application) showed synergistic toxic effect on Coprinus cinereus oidia particulary at the high concentration (50 pl/rnl) before mutagenic drug application and tow concentration (40 and 50 ul/rnl) after mutagenic drug application. On other hand, the lower concentration 20 and 30 ~lIml showed a significant increase in the protective effect of mutagenic level in HGPRT gene, in both treatment before and after the mutagenic dru ; application, it could be possible that the use of volatile oil of Sa/via fruticosa plant a. noderate concentration
more effective in preventing mutagenicity